chromatin immunoprecipitation chip assay simple chip plus sonication chromatin ip kit (Cell Signaling Technology Inc)
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chromatin immunoprecipitation chip assay simple chip plus sonication chromatin ip kit
Chromatin Immunoprecipitation Chip Assay Simple Chip Plus Sonication Chromatin Ip Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 734 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sonication+chromatin+ip+kit/SimpleChIP+Plus+Sonication+Chromatin+IP+Kit/pm42790710-133-0-12
Average 97 stars, based on 734 article reviews
Chromatin Immunoprecipitation Chip Assay Simple Chip Plus Sonication Chromatin Ip Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 734 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sonication+chromatin+ip+kit/SimpleChIP+Plus+Sonication+Chromatin+IP+Kit/pm42790710-133-0-12
Average 97 stars, based on 734 article reviews
chromatin immunoprecipitation chip assay simple chip plus sonication chromatin ip kit - by Bioz Stars,
2026-10
97/100 stars
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Sonication:Article Title: ID1 expressing macrophages support cancer cell stemness and limit CD8 + T cell infiltration in colorectal cancer Article Snippet: The following commercial kits were used: CFSE cell division tracker kit (Biolegend, 423801), Precision count beadsTM (Biolegend, 424902), SimpleChIP® plus sonication chromatin IP kit (Cell Signaling Technology, 56383), Four color labeled kit (TSA-RM) (PANOVUE Biotechnology Co.,LTD, 1100020), CD8 + T cell isolation kit, human (Miltenyi Biotec, 130-096-495), CD8 + T cells isolation kit, mouse (Miltenyl Biotec, 130-104-075), RNA-quick purification kit (Shang Hai Yishan Biotechnology Co., LTD, ES-RN001), Anti-PE microbeads (Miltenyi Biotec, 130-048-801), CD326 (EpCAM) microbeads, mouse (Miltenyi Biotec, 130-105-958), Cytofix/CytopermTM fixation/permeabilization solution kit (BD Biosciences, 554714), TransScrip@ One-Step RT-PCR superMix (Transgene, AT411-02), Secrete-pair gaussia luciferase assay kit (GeneCopoeia, LF062), Duolink® in situ detection reagents red (Merck, DUO92008), Mouse C–C motif chemokine 4 (Ccl4) ELISA kit (4A Biotech, MOEB0088), Human C–C motif chemokine 4 (CCL4) ELISA kit (4A Biotech, HUEB0132), Mouse plasminogen activator inhibitor 2, macrophage (Serpinb2) ELISA kit (4A Biotech, MOEB1945), Human plasminogen activator inhibitor 2 (SerpinB2) ELISA Kit (4A Biotech, HUEB2195), MycoBlue Mycoplasma Detector (Vazyme, D101-02), Dual luciferase reporter assay system (Promega, E1910), ALDEFLUORTM Kit (STEMCELL Technologies, 01700). .. The following commercial kits were used: CFSE cell division tracker kit (Biolegend, 423801), Precision count beadsTM (Biolegend, 424902), SimpleChIP® plus Article Title: PJA2 suppresses colorectal cancer progression by controlling HDAC2 degradation and stability. Article Snippet: .. Chromatin immunoprecipitation assays (CHIP) The Article Title: Helicobacter pylori Exploit Short-Chain Fatty Acids-Induced CAPZA1 Overexpression to Emerge CD44v9-Positive Stemness Article Snippet: HDAC activity was measured using an HDAC Activity Assay kit (BioVision, Milpitas, CA, cat# K331). .. Immunoprecipitation was performed using a Simple Chromatin Immunoprecipitation (ChIP) Plus Article Title: ID1 expressing macrophages support cancer cell stemness and limit CD8 + T cell infiltration in colorectal cancer. Article Snippet: The following chemicals and recombinant proteins were used: Y15 (Selleck, S5321), 5-Fluorouracil (5-FU) (Selleck, S1209), ML323 (Selleck, S7529), Propidium iodide (Solarbio, P8080), Matrigel (Corning, 354230), Fibronectin (Corning, 354008), StemXVivo serum-free tumorsphere media (R&D, CCM012), Ionomycin (Merck, IO634), GolgiStop (BD Biosciences, 554724), Brefeldin A solution (1,000X) Nature Communications | (2023) 14:7661 14 (Biolegend, 4200601), β-Mercaptoethanol (Solarbio, M8210), Human truStain FcXTM (Fc receptor blocking solution) (Biolegend, 422302), Recombinantmurine IL-4 (Peprotech, 214-14), Recombinantmurine IL2 (Peprotech, 212-12), Recombinant human IFN-γ (Peprotech, 300-02), Recombinant murine IFN-γ (Peprotech, 315-05), Recombinant murine m-CSF (Peprotech, 315-02), Leptomycin B (Selleck, S7580), Phorbol 12- myristate 13-acetate (PMA) (Merck, P8139), Collagenase IV (Merck, C4BIOC), Dnase I (Merck, 10104159001), Lithium phosphorus sulfide (LPS) powder (Merck, 916374), Puromycin (Invitrogen, A1113803), Fluorescent mounting medium with DAPI (ZSGB-BIO, ZLI-9557), Lymphocyte separation medium (Solarbio, P8900), 2,2,2-tribromoethanol (Merck, T48402), Protein A/G Plus-agarose (Santa Cruz, sc-2003), Ovalbumin (257-264) chicken (Merck, S7951), Verteporfin (Selleck, S1786). .. The following commercial kits were used: CFSE cell division tracker kit (Biolegend, 423801), Precision count beadsTM (Biolegend, 424902), SimpleChIP® plus Article Title: Knockdown of ZBTB11 impedes R‐loop elimination and increases the sensitivity to cisplatin by inhibiting DDX1 transcription in bladder cancer Article Snippet: .. Plus Article Title: Propofol protects against high glucose-mediated endothelial injury via inhibition of COX2 and iNOS expressions Article Snippet: .. ChIP assays were carried out using a Simple Chromatin immunoprecipitation (ChIP) Plus Article Title: PJA2 Suppresses Colorectal Cancer Progression by Controlling HDAC2 Degradation and Stability Article Snippet: .. The Chromatin Immunoprecipitation:Article Title: ID1 expressing macrophages support cancer cell stemness and limit CD8 + T cell infiltration in colorectal cancer Article Snippet: The following commercial kits were used: CFSE cell division tracker kit (Biolegend, 423801), Precision count beadsTM (Biolegend, 424902), SimpleChIP® plus sonication chromatin IP kit (Cell Signaling Technology, 56383), Four color labeled kit (TSA-RM) (PANOVUE Biotechnology Co.,LTD, 1100020), CD8 + T cell isolation kit, human (Miltenyi Biotec, 130-096-495), CD8 + T cells isolation kit, mouse (Miltenyl Biotec, 130-104-075), RNA-quick purification kit (Shang Hai Yishan Biotechnology Co., LTD, ES-RN001), Anti-PE microbeads (Miltenyi Biotec, 130-048-801), CD326 (EpCAM) microbeads, mouse (Miltenyi Biotec, 130-105-958), Cytofix/CytopermTM fixation/permeabilization solution kit (BD Biosciences, 554714), TransScrip@ One-Step RT-PCR superMix (Transgene, AT411-02), Secrete-pair gaussia luciferase assay kit (GeneCopoeia, LF062), Duolink® in situ detection reagents red (Merck, DUO92008), Mouse C–C motif chemokine 4 (Ccl4) ELISA kit (4A Biotech, MOEB0088), Human C–C motif chemokine 4 (CCL4) ELISA kit (4A Biotech, HUEB0132), Mouse plasminogen activator inhibitor 2, macrophage (Serpinb2) ELISA kit (4A Biotech, MOEB1945), Human plasminogen activator inhibitor 2 (SerpinB2) ELISA Kit (4A Biotech, HUEB2195), MycoBlue Mycoplasma Detector (Vazyme, D101-02), Dual luciferase reporter assay system (Promega, E1910), ALDEFLUORTM Kit (STEMCELL Technologies, 01700). .. The following commercial kits were used: CFSE cell division tracker kit (Biolegend, 423801), Precision count beadsTM (Biolegend, 424902), SimpleChIP® plus Article Title: PJA2 suppresses colorectal cancer progression by controlling HDAC2 degradation and stability. Article Snippet: .. Chromatin immunoprecipitation assays (CHIP) The Article Title: Helicobacter pylori Exploit Short-Chain Fatty Acids-Induced CAPZA1 Overexpression to Emerge CD44v9-Positive Stemness Article Snippet: HDAC activity was measured using an HDAC Activity Assay kit (BioVision, Milpitas, CA, cat# K331). .. Immunoprecipitation was performed using a Simple Chromatin Immunoprecipitation (ChIP) Plus Article Title: ID1 expressing macrophages support cancer cell stemness and limit CD8 + T cell infiltration in colorectal cancer. Article Snippet: The following chemicals and recombinant proteins were used: Y15 (Selleck, S5321), 5-Fluorouracil (5-FU) (Selleck, S1209), ML323 (Selleck, S7529), Propidium iodide (Solarbio, P8080), Matrigel (Corning, 354230), Fibronectin (Corning, 354008), StemXVivo serum-free tumorsphere media (R&D, CCM012), Ionomycin (Merck, IO634), GolgiStop (BD Biosciences, 554724), Brefeldin A solution (1,000X) Nature Communications | (2023) 14:7661 14 (Biolegend, 4200601), β-Mercaptoethanol (Solarbio, M8210), Human truStain FcXTM (Fc receptor blocking solution) (Biolegend, 422302), Recombinantmurine IL-4 (Peprotech, 214-14), Recombinantmurine IL2 (Peprotech, 212-12), Recombinant human IFN-γ (Peprotech, 300-02), Recombinant murine IFN-γ (Peprotech, 315-05), Recombinant murine m-CSF (Peprotech, 315-02), Leptomycin B (Selleck, S7580), Phorbol 12- myristate 13-acetate (PMA) (Merck, P8139), Collagenase IV (Merck, C4BIOC), Dnase I (Merck, 10104159001), Lithium phosphorus sulfide (LPS) powder (Merck, 916374), Puromycin (Invitrogen, A1113803), Fluorescent mounting medium with DAPI (ZSGB-BIO, ZLI-9557), Lymphocyte separation medium (Solarbio, P8900), 2,2,2-tribromoethanol (Merck, T48402), Protein A/G Plus-agarose (Santa Cruz, sc-2003), Ovalbumin (257-264) chicken (Merck, S7951), Verteporfin (Selleck, S1786). .. The following commercial kits were used: CFSE cell division tracker kit (Biolegend, 423801), Precision count beadsTM (Biolegend, 424902), SimpleChIP® plus Article Title: Knockdown of ZBTB11 impedes R‐loop elimination and increases the sensitivity to cisplatin by inhibiting DDX1 transcription in bladder cancer Article Snippet: .. Plus Article Title: Propofol protects against high glucose-mediated endothelial injury via inhibition of COX2 and iNOS expressions Article Snippet: .. ChIP assays were carried out using a Simple Chromatin immunoprecipitation (ChIP) Plus Article Title: PJA2 Suppresses Colorectal Cancer Progression by Controlling HDAC2 Degradation and Stability Article Snippet: .. The Labeling:Article Title: ID1 expressing macrophages support cancer cell stemness and limit CD8 + T cell infiltration in colorectal cancer Article Snippet: The following commercial kits were used: CFSE cell division tracker kit (Biolegend, 423801), Precision count beadsTM (Biolegend, 424902), SimpleChIP® plus sonication chromatin IP kit (Cell Signaling Technology, 56383), Four color labeled kit (TSA-RM) (PANOVUE Biotechnology Co.,LTD, 1100020), CD8 + T cell isolation kit, human (Miltenyi Biotec, 130-096-495), CD8 + T cells isolation kit, mouse (Miltenyl Biotec, 130-104-075), RNA-quick purification kit (Shang Hai Yishan Biotechnology Co., LTD, ES-RN001), Anti-PE microbeads (Miltenyi Biotec, 130-048-801), CD326 (EpCAM) microbeads, mouse (Miltenyi Biotec, 130-105-958), Cytofix/CytopermTM fixation/permeabilization solution kit (BD Biosciences, 554714), TransScrip@ One-Step RT-PCR superMix (Transgene, AT411-02), Secrete-pair gaussia luciferase assay kit (GeneCopoeia, LF062), Duolink® in situ detection reagents red (Merck, DUO92008), Mouse C–C motif chemokine 4 (Ccl4) ELISA kit (4A Biotech, MOEB0088), Human C–C motif chemokine 4 (CCL4) ELISA kit (4A Biotech, HUEB0132), Mouse plasminogen activator inhibitor 2, macrophage (Serpinb2) ELISA kit (4A Biotech, MOEB1945), Human plasminogen activator inhibitor 2 (SerpinB2) ELISA Kit (4A Biotech, HUEB2195), MycoBlue Mycoplasma Detector (Vazyme, D101-02), Dual luciferase reporter assay system (Promega, E1910), ALDEFLUORTM Kit (STEMCELL Technologies, 01700). .. The following commercial kits were used: CFSE cell division tracker kit (Biolegend, 423801), Precision count beadsTM (Biolegend, 424902), SimpleChIP® plus Article Title: ID1 expressing macrophages support cancer cell stemness and limit CD8 + T cell infiltration in colorectal cancer. Article Snippet: The following chemicals and recombinant proteins were used: Y15 (Selleck, S5321), 5-Fluorouracil (5-FU) (Selleck, S1209), ML323 (Selleck, S7529), Propidium iodide (Solarbio, P8080), Matrigel (Corning, 354230), Fibronectin (Corning, 354008), StemXVivo serum-free tumorsphere media (R&D, CCM012), Ionomycin (Merck, IO634), GolgiStop (BD Biosciences, 554724), Brefeldin A solution (1,000X) Nature Communications | (2023) 14:7661 14 (Biolegend, 4200601), β-Mercaptoethanol (Solarbio, M8210), Human truStain FcXTM (Fc receptor blocking solution) (Biolegend, 422302), Recombinantmurine IL-4 (Peprotech, 214-14), Recombinantmurine IL2 (Peprotech, 212-12), Recombinant human IFN-γ (Peprotech, 300-02), Recombinant murine IFN-γ (Peprotech, 315-05), Recombinant murine m-CSF (Peprotech, 315-02), Leptomycin B (Selleck, S7580), Phorbol 12- myristate 13-acetate (PMA) (Merck, P8139), Collagenase IV (Merck, C4BIOC), Dnase I (Merck, 10104159001), Lithium phosphorus sulfide (LPS) powder (Merck, 916374), Puromycin (Invitrogen, A1113803), Fluorescent mounting medium with DAPI (ZSGB-BIO, ZLI-9557), Lymphocyte separation medium (Solarbio, P8900), 2,2,2-tribromoethanol (Merck, T48402), Protein A/G Plus-agarose (Santa Cruz, sc-2003), Ovalbumin (257-264) chicken (Merck, S7951), Verteporfin (Selleck, S1786). .. The following commercial kits were used: CFSE cell division tracker kit (Biolegend, 423801), Precision count beadsTM (Biolegend, 424902), SimpleChIP® plus Cell Isolation:Article Title: ID1 expressing macrophages support cancer cell stemness and limit CD8 + T cell infiltration in colorectal cancer Article Snippet: The following commercial kits were used: CFSE cell division tracker kit (Biolegend, 423801), Precision count beadsTM (Biolegend, 424902), SimpleChIP® plus sonication chromatin IP kit (Cell Signaling Technology, 56383), Four color labeled kit (TSA-RM) (PANOVUE Biotechnology Co.,LTD, 1100020), CD8 + T cell isolation kit, human (Miltenyi Biotec, 130-096-495), CD8 + T cells isolation kit, mouse (Miltenyl Biotec, 130-104-075), RNA-quick purification kit (Shang Hai Yishan Biotechnology Co., LTD, ES-RN001), Anti-PE microbeads (Miltenyi Biotec, 130-048-801), CD326 (EpCAM) microbeads, mouse (Miltenyi Biotec, 130-105-958), Cytofix/CytopermTM fixation/permeabilization solution kit (BD Biosciences, 554714), TransScrip@ One-Step RT-PCR superMix (Transgene, AT411-02), Secrete-pair gaussia luciferase assay kit (GeneCopoeia, LF062), Duolink® in situ detection reagents red (Merck, DUO92008), Mouse C–C motif chemokine 4 (Ccl4) ELISA kit (4A Biotech, MOEB0088), Human C–C motif chemokine 4 (CCL4) ELISA kit (4A Biotech, HUEB0132), Mouse plasminogen activator inhibitor 2, macrophage (Serpinb2) ELISA kit (4A Biotech, MOEB1945), Human plasminogen activator inhibitor 2 (SerpinB2) ELISA Kit (4A Biotech, HUEB2195), MycoBlue Mycoplasma Detector (Vazyme, D101-02), Dual luciferase reporter assay system (Promega, E1910), ALDEFLUORTM Kit (STEMCELL Technologies, 01700). .. The following commercial kits were used: CFSE cell division tracker kit (Biolegend, 423801), Precision count beadsTM (Biolegend, 424902), SimpleChIP® plus Article Title: ID1 expressing macrophages support cancer cell stemness and limit CD8 + T cell infiltration in colorectal cancer. Article Snippet: The following chemicals and recombinant proteins were used: Y15 (Selleck, S5321), 5-Fluorouracil (5-FU) (Selleck, S1209), ML323 (Selleck, S7529), Propidium iodide (Solarbio, P8080), Matrigel (Corning, 354230), Fibronectin (Corning, 354008), StemXVivo serum-free tumorsphere media (R&D, CCM012), Ionomycin (Merck, IO634), GolgiStop (BD Biosciences, 554724), Brefeldin A solution (1,000X) Nature Communications | (2023) 14:7661 14 (Biolegend, 4200601), β-Mercaptoethanol (Solarbio, M8210), Human truStain FcXTM (Fc receptor blocking solution) (Biolegend, 422302), Recombinantmurine IL-4 (Peprotech, 214-14), Recombinantmurine IL2 (Peprotech, 212-12), Recombinant human IFN-γ (Peprotech, 300-02), Recombinant murine IFN-γ (Peprotech, 315-05), Recombinant murine m-CSF (Peprotech, 315-02), Leptomycin B (Selleck, S7580), Phorbol 12- myristate 13-acetate (PMA) (Merck, P8139), Collagenase IV (Merck, C4BIOC), Dnase I (Merck, 10104159001), Lithium phosphorus sulfide (LPS) powder (Merck, 916374), Puromycin (Invitrogen, A1113803), Fluorescent mounting medium with DAPI (ZSGB-BIO, ZLI-9557), Lymphocyte separation medium (Solarbio, P8900), 2,2,2-tribromoethanol (Merck, T48402), Protein A/G Plus-agarose (Santa Cruz, sc-2003), Ovalbumin (257-264) chicken (Merck, S7951), Verteporfin (Selleck, S1786). .. The following commercial kits were used: CFSE cell division tracker kit (Biolegend, 423801), Precision count beadsTM (Biolegend, 424902), SimpleChIP® plus Isolation:Article Title: ID1 expressing macrophages support cancer cell stemness and limit CD8 + T cell infiltration in colorectal cancer Article Snippet: The following commercial kits were used: CFSE cell division tracker kit (Biolegend, 423801), Precision count beadsTM (Biolegend, 424902), SimpleChIP® plus sonication chromatin IP kit (Cell Signaling Technology, 56383), Four color labeled kit (TSA-RM) (PANOVUE Biotechnology Co.,LTD, 1100020), CD8 + T cell isolation kit, human (Miltenyi Biotec, 130-096-495), CD8 + T cells isolation kit, mouse (Miltenyl Biotec, 130-104-075), RNA-quick purification kit (Shang Hai Yishan Biotechnology Co., LTD, ES-RN001), Anti-PE microbeads (Miltenyi Biotec, 130-048-801), CD326 (EpCAM) microbeads, mouse (Miltenyi Biotec, 130-105-958), Cytofix/CytopermTM fixation/permeabilization solution kit (BD Biosciences, 554714), TransScrip@ One-Step RT-PCR superMix (Transgene, AT411-02), Secrete-pair gaussia luciferase assay kit (GeneCopoeia, LF062), Duolink® in situ detection reagents red (Merck, DUO92008), Mouse C–C motif chemokine 4 (Ccl4) ELISA kit (4A Biotech, MOEB0088), Human C–C motif chemokine 4 (CCL4) ELISA kit (4A Biotech, HUEB0132), Mouse plasminogen activator inhibitor 2, macrophage (Serpinb2) ELISA kit (4A Biotech, MOEB1945), Human plasminogen activator inhibitor 2 (SerpinB2) ELISA Kit (4A Biotech, HUEB2195), MycoBlue Mycoplasma Detector (Vazyme, D101-02), Dual luciferase reporter assay system (Promega, E1910), ALDEFLUORTM Kit (STEMCELL Technologies, 01700). .. The following commercial kits were used: CFSE cell division tracker kit (Biolegend, 423801), Precision count beadsTM (Biolegend, 424902), SimpleChIP® plus Article Title: ID1 expressing macrophages support cancer cell stemness and limit CD8 + T cell infiltration in colorectal cancer. Article Snippet: The following chemicals and recombinant proteins were used: Y15 (Selleck, S5321), 5-Fluorouracil (5-FU) (Selleck, S1209), ML323 (Selleck, S7529), Propidium iodide (Solarbio, P8080), Matrigel (Corning, 354230), Fibronectin (Corning, 354008), StemXVivo serum-free tumorsphere media (R&D, CCM012), Ionomycin (Merck, IO634), GolgiStop (BD Biosciences, 554724), Brefeldin A solution (1,000X) Nature Communications | (2023) 14:7661 14 (Biolegend, 4200601), β-Mercaptoethanol (Solarbio, M8210), Human truStain FcXTM (Fc receptor blocking solution) (Biolegend, 422302), Recombinantmurine IL-4 (Peprotech, 214-14), Recombinantmurine IL2 (Peprotech, 212-12), Recombinant human IFN-γ (Peprotech, 300-02), Recombinant murine IFN-γ (Peprotech, 315-05), Recombinant murine m-CSF (Peprotech, 315-02), Leptomycin B (Selleck, S7580), Phorbol 12- myristate 13-acetate (PMA) (Merck, P8139), Collagenase IV (Merck, C4BIOC), Dnase I (Merck, 10104159001), Lithium phosphorus sulfide (LPS) powder (Merck, 916374), Puromycin (Invitrogen, A1113803), Fluorescent mounting medium with DAPI (ZSGB-BIO, ZLI-9557), Lymphocyte separation medium (Solarbio, P8900), 2,2,2-tribromoethanol (Merck, T48402), Protein A/G Plus-agarose (Santa Cruz, sc-2003), Ovalbumin (257-264) chicken (Merck, S7951), Verteporfin (Selleck, S1786). .. The following commercial kits were used: CFSE cell division tracker kit (Biolegend, 423801), Precision count beadsTM (Biolegend, 424902), SimpleChIP® plus Purification:Article Title: ID1 expressing macrophages support cancer cell stemness and limit CD8 + T cell infiltration in colorectal cancer Article Snippet: The following commercial kits were used: CFSE cell division tracker kit (Biolegend, 423801), Precision count beadsTM (Biolegend, 424902), SimpleChIP® plus sonication chromatin IP kit (Cell Signaling Technology, 56383), Four color labeled kit (TSA-RM) (PANOVUE Biotechnology Co.,LTD, 1100020), CD8 + T cell isolation kit, human (Miltenyi Biotec, 130-096-495), CD8 + T cells isolation kit, mouse (Miltenyl Biotec, 130-104-075), RNA-quick purification kit (Shang Hai Yishan Biotechnology Co., LTD, ES-RN001), Anti-PE microbeads (Miltenyi Biotec, 130-048-801), CD326 (EpCAM) microbeads, mouse (Miltenyi Biotec, 130-105-958), Cytofix/CytopermTM fixation/permeabilization solution kit (BD Biosciences, 554714), TransScrip@ One-Step RT-PCR superMix (Transgene, AT411-02), Secrete-pair gaussia luciferase assay kit (GeneCopoeia, LF062), Duolink® in situ detection reagents red (Merck, DUO92008), Mouse C–C motif chemokine 4 (Ccl4) ELISA kit (4A Biotech, MOEB0088), Human C–C motif chemokine 4 (CCL4) ELISA kit (4A Biotech, HUEB0132), Mouse plasminogen activator inhibitor 2, macrophage (Serpinb2) ELISA kit (4A Biotech, MOEB1945), Human plasminogen activator inhibitor 2 (SerpinB2) ELISA Kit (4A Biotech, HUEB2195), MycoBlue Mycoplasma Detector (Vazyme, D101-02), Dual luciferase reporter assay system (Promega, E1910), ALDEFLUORTM Kit (STEMCELL Technologies, 01700). .. The following commercial kits were used: CFSE cell division tracker kit (Biolegend, 423801), Precision count beadsTM (Biolegend, 424902), SimpleChIP® plus Article Title: ID1 expressing macrophages support cancer cell stemness and limit CD8 + T cell infiltration in colorectal cancer. Article Snippet: The following chemicals and recombinant proteins were used: Y15 (Selleck, S5321), 5-Fluorouracil (5-FU) (Selleck, S1209), ML323 (Selleck, S7529), Propidium iodide (Solarbio, P8080), Matrigel (Corning, 354230), Fibronectin (Corning, 354008), StemXVivo serum-free tumorsphere media (R&D, CCM012), Ionomycin (Merck, IO634), GolgiStop (BD Biosciences, 554724), Brefeldin A solution (1,000X) Nature Communications | (2023) 14:7661 14 (Biolegend, 4200601), β-Mercaptoethanol (Solarbio, M8210), Human truStain FcXTM (Fc receptor blocking solution) (Biolegend, 422302), Recombinantmurine IL-4 (Peprotech, 214-14), Recombinantmurine IL2 (Peprotech, 212-12), Recombinant human IFN-γ (Peprotech, 300-02), Recombinant murine IFN-γ (Peprotech, 315-05), Recombinant murine m-CSF (Peprotech, 315-02), Leptomycin B (Selleck, S7580), Phorbol 12- myristate 13-acetate (PMA) (Merck, P8139), Collagenase IV (Merck, C4BIOC), Dnase I (Merck, 10104159001), Lithium phosphorus sulfide (LPS) powder (Merck, 916374), Puromycin (Invitrogen, A1113803), Fluorescent mounting medium with DAPI (ZSGB-BIO, ZLI-9557), Lymphocyte separation medium (Solarbio, P8900), 2,2,2-tribromoethanol (Merck, T48402), Protein A/G Plus-agarose (Santa Cruz, sc-2003), Ovalbumin (257-264) chicken (Merck, S7951), Verteporfin (Selleck, S1786). .. The following commercial kits were used: CFSE cell division tracker kit (Biolegend, 423801), Precision count beadsTM (Biolegend, 424902), SimpleChIP® plus One Step RT-PCR:Article Title: ID1 expressing macrophages support cancer cell stemness and limit CD8 + T cell infiltration in colorectal cancer Article Snippet: The following commercial kits were used: CFSE cell division tracker kit (Biolegend, 423801), Precision count beadsTM (Biolegend, 424902), SimpleChIP® plus sonication chromatin IP kit (Cell Signaling Technology, 56383), Four color labeled kit (TSA-RM) (PANOVUE Biotechnology Co.,LTD, 1100020), CD8 + T cell isolation kit, human (Miltenyi Biotec, 130-096-495), CD8 + T cells isolation kit, mouse (Miltenyl Biotec, 130-104-075), RNA-quick purification kit (Shang Hai Yishan Biotechnology Co., LTD, ES-RN001), Anti-PE microbeads (Miltenyi Biotec, 130-048-801), CD326 (EpCAM) microbeads, mouse (Miltenyi Biotec, 130-105-958), Cytofix/CytopermTM fixation/permeabilization solution kit (BD Biosciences, 554714), TransScrip@ One-Step RT-PCR superMix (Transgene, AT411-02), Secrete-pair gaussia luciferase assay kit (GeneCopoeia, LF062), Duolink® in situ detection reagents red (Merck, DUO92008), Mouse C–C motif chemokine 4 (Ccl4) ELISA kit (4A Biotech, MOEB0088), Human C–C motif chemokine 4 (CCL4) ELISA kit (4A Biotech, HUEB0132), Mouse plasminogen activator inhibitor 2, macrophage (Serpinb2) ELISA kit (4A Biotech, MOEB1945), Human plasminogen activator inhibitor 2 (SerpinB2) ELISA Kit (4A Biotech, HUEB2195), MycoBlue Mycoplasma Detector (Vazyme, D101-02), Dual luciferase reporter assay system (Promega, E1910), ALDEFLUORTM Kit (STEMCELL Technologies, 01700). .. The following commercial kits were used: CFSE cell division tracker kit (Biolegend, 423801), Precision count beadsTM (Biolegend, 424902), SimpleChIP® plus Article Title: ID1 expressing macrophages support cancer cell stemness and limit CD8 + T cell infiltration in colorectal cancer. Article Snippet: The following chemicals and recombinant proteins were used: Y15 (Selleck, S5321), 5-Fluorouracil (5-FU) (Selleck, S1209), ML323 (Selleck, S7529), Propidium iodide (Solarbio, P8080), Matrigel (Corning, 354230), Fibronectin (Corning, 354008), StemXVivo serum-free tumorsphere media (R&D, CCM012), Ionomycin (Merck, IO634), GolgiStop (BD Biosciences, 554724), Brefeldin A solution (1,000X) Nature Communications | (2023) 14:7661 14 (Biolegend, 4200601), β-Mercaptoethanol (Solarbio, M8210), Human truStain FcXTM (Fc receptor blocking solution) (Biolegend, 422302), Recombinantmurine IL-4 (Peprotech, 214-14), Recombinantmurine IL2 (Peprotech, 212-12), Recombinant human IFN-γ (Peprotech, 300-02), Recombinant murine IFN-γ (Peprotech, 315-05), Recombinant murine m-CSF (Peprotech, 315-02), Leptomycin B (Selleck, S7580), Phorbol 12- myristate 13-acetate (PMA) (Merck, P8139), Collagenase IV (Merck, C4BIOC), Dnase I (Merck, 10104159001), Lithium phosphorus sulfide (LPS) powder (Merck, 916374), Puromycin (Invitrogen, A1113803), Fluorescent mounting medium with DAPI (ZSGB-BIO, ZLI-9557), Lymphocyte separation medium (Solarbio, P8900), 2,2,2-tribromoethanol (Merck, T48402), Protein A/G Plus-agarose (Santa Cruz, sc-2003), Ovalbumin (257-264) chicken (Merck, S7951), Verteporfin (Selleck, S1786). .. The following commercial kits were used: CFSE cell division tracker kit (Biolegend, 423801), Precision count beadsTM (Biolegend, 424902), SimpleChIP® plus Luciferase:Article Title: ID1 expressing macrophages support cancer cell stemness and limit CD8 + T cell infiltration in colorectal cancer Article Snippet: The following commercial kits were used: CFSE cell division tracker kit (Biolegend, 423801), Precision count beadsTM (Biolegend, 424902), SimpleChIP® plus sonication chromatin IP kit (Cell Signaling Technology, 56383), Four color labeled kit (TSA-RM) (PANOVUE Biotechnology Co.,LTD, 1100020), CD8 + T cell isolation kit, human (Miltenyi Biotec, 130-096-495), CD8 + T cells isolation kit, mouse (Miltenyl Biotec, 130-104-075), RNA-quick purification kit (Shang Hai Yishan Biotechnology Co., LTD, ES-RN001), Anti-PE microbeads (Miltenyi Biotec, 130-048-801), CD326 (EpCAM) microbeads, mouse (Miltenyi Biotec, 130-105-958), Cytofix/CytopermTM fixation/permeabilization solution kit (BD Biosciences, 554714), TransScrip@ One-Step RT-PCR superMix (Transgene, AT411-02), Secrete-pair gaussia luciferase assay kit (GeneCopoeia, LF062), Duolink® in situ detection reagents red (Merck, DUO92008), Mouse C–C motif chemokine 4 (Ccl4) ELISA kit (4A Biotech, MOEB0088), Human C–C motif chemokine 4 (CCL4) ELISA kit (4A Biotech, HUEB0132), Mouse plasminogen activator inhibitor 2, macrophage (Serpinb2) ELISA kit (4A Biotech, MOEB1945), Human plasminogen activator inhibitor 2 (SerpinB2) ELISA Kit (4A Biotech, HUEB2195), MycoBlue Mycoplasma Detector (Vazyme, D101-02), Dual luciferase reporter assay system (Promega, E1910), ALDEFLUORTM Kit (STEMCELL Technologies, 01700). .. The following commercial kits were used: CFSE cell division tracker kit (Biolegend, 423801), Precision count beadsTM (Biolegend, 424902), SimpleChIP® plus Article Title: ID1 expressing macrophages support cancer cell stemness and limit CD8 + T cell infiltration in colorectal cancer. Article Snippet: The following chemicals and recombinant proteins were used: Y15 (Selleck, S5321), 5-Fluorouracil (5-FU) (Selleck, S1209), ML323 (Selleck, S7529), Propidium iodide (Solarbio, P8080), Matrigel (Corning, 354230), Fibronectin (Corning, 354008), StemXVivo serum-free tumorsphere media (R&D, CCM012), Ionomycin (Merck, IO634), GolgiStop (BD Biosciences, 554724), Brefeldin A solution (1,000X) Nature Communications | (2023) 14:7661 14 (Biolegend, 4200601), β-Mercaptoethanol (Solarbio, M8210), Human truStain FcXTM (Fc receptor blocking solution) (Biolegend, 422302), Recombinantmurine IL-4 (Peprotech, 214-14), Recombinantmurine IL2 (Peprotech, 212-12), Recombinant human IFN-γ (Peprotech, 300-02), Recombinant murine IFN-γ (Peprotech, 315-05), Recombinant murine m-CSF (Peprotech, 315-02), Leptomycin B (Selleck, S7580), Phorbol 12- myristate 13-acetate (PMA) (Merck, P8139), Collagenase IV (Merck, C4BIOC), Dnase I (Merck, 10104159001), Lithium phosphorus sulfide (LPS) powder (Merck, 916374), Puromycin (Invitrogen, A1113803), Fluorescent mounting medium with DAPI (ZSGB-BIO, ZLI-9557), Lymphocyte separation medium (Solarbio, P8900), 2,2,2-tribromoethanol (Merck, T48402), Protein A/G Plus-agarose (Santa Cruz, sc-2003), Ovalbumin (257-264) chicken (Merck, S7951), Verteporfin (Selleck, S1786). .. The following commercial kits were used: CFSE cell division tracker kit (Biolegend, 423801), Precision count beadsTM (Biolegend, 424902), SimpleChIP® plus In Situ:Article Title: ID1 expressing macrophages support cancer cell stemness and limit CD8 + T cell infiltration in colorectal cancer Article Snippet: The following commercial kits were used: CFSE cell division tracker kit (Biolegend, 423801), Precision count beadsTM (Biolegend, 424902), SimpleChIP® plus sonication chromatin IP kit (Cell Signaling Technology, 56383), Four color labeled kit (TSA-RM) (PANOVUE Biotechnology Co.,LTD, 1100020), CD8 + T cell isolation kit, human (Miltenyi Biotec, 130-096-495), CD8 + T cells isolation kit, mouse (Miltenyl Biotec, 130-104-075), RNA-quick purification kit (Shang Hai Yishan Biotechnology Co., LTD, ES-RN001), Anti-PE microbeads (Miltenyi Biotec, 130-048-801), CD326 (EpCAM) microbeads, mouse (Miltenyi Biotec, 130-105-958), Cytofix/CytopermTM fixation/permeabilization solution kit (BD Biosciences, 554714), TransScrip@ One-Step RT-PCR superMix (Transgene, AT411-02), Secrete-pair gaussia luciferase assay kit (GeneCopoeia, LF062), Duolink® in situ detection reagents red (Merck, DUO92008), Mouse C–C motif chemokine 4 (Ccl4) ELISA kit (4A Biotech, MOEB0088), Human C–C motif chemokine 4 (CCL4) ELISA kit (4A Biotech, HUEB0132), Mouse plasminogen activator inhibitor 2, macrophage (Serpinb2) ELISA kit (4A Biotech, MOEB1945), Human plasminogen activator inhibitor 2 (SerpinB2) ELISA Kit (4A Biotech, HUEB2195), MycoBlue Mycoplasma Detector (Vazyme, D101-02), Dual luciferase reporter assay system (Promega, E1910), ALDEFLUORTM Kit (STEMCELL Technologies, 01700). .. The following commercial kits were used: CFSE cell division tracker kit (Biolegend, 423801), Precision count beadsTM (Biolegend, 424902), SimpleChIP® plus Article Title: ID1 expressing macrophages support cancer cell stemness and limit CD8 + T cell infiltration in colorectal cancer. Article Snippet: The following chemicals and recombinant proteins were used: Y15 (Selleck, S5321), 5-Fluorouracil (5-FU) (Selleck, S1209), ML323 (Selleck, S7529), Propidium iodide (Solarbio, P8080), Matrigel (Corning, 354230), Fibronectin (Corning, 354008), StemXVivo serum-free tumorsphere media (R&D, CCM012), Ionomycin (Merck, IO634), GolgiStop (BD Biosciences, 554724), Brefeldin A solution (1,000X) Nature Communications | (2023) 14:7661 14 (Biolegend, 4200601), β-Mercaptoethanol (Solarbio, M8210), Human truStain FcXTM (Fc receptor blocking solution) (Biolegend, 422302), Recombinantmurine IL-4 (Peprotech, 214-14), Recombinantmurine IL2 (Peprotech, 212-12), Recombinant human IFN-γ (Peprotech, 300-02), Recombinant murine IFN-γ (Peprotech, 315-05), Recombinant murine m-CSF (Peprotech, 315-02), Leptomycin B (Selleck, S7580), Phorbol 12- myristate 13-acetate (PMA) (Merck, P8139), Collagenase IV (Merck, C4BIOC), Dnase I (Merck, 10104159001), Lithium phosphorus sulfide (LPS) powder (Merck, 916374), Puromycin (Invitrogen, A1113803), Fluorescent mounting medium with DAPI (ZSGB-BIO, ZLI-9557), Lymphocyte separation medium (Solarbio, P8900), 2,2,2-tribromoethanol (Merck, T48402), Protein A/G Plus-agarose (Santa Cruz, sc-2003), Ovalbumin (257-264) chicken (Merck, S7951), Verteporfin (Selleck, S1786). .. The following commercial kits were used: CFSE cell division tracker kit (Biolegend, 423801), Precision count beadsTM (Biolegend, 424902), SimpleChIP® plus Enzyme-linked Immunosorbent Assay:Article Title: ID1 expressing macrophages support cancer cell stemness and limit CD8 + T cell infiltration in colorectal cancer Article Snippet: The following commercial kits were used: CFSE cell division tracker kit (Biolegend, 423801), Precision count beadsTM (Biolegend, 424902), SimpleChIP® plus sonication chromatin IP kit (Cell Signaling Technology, 56383), Four color labeled kit (TSA-RM) (PANOVUE Biotechnology Co.,LTD, 1100020), CD8 + T cell isolation kit, human (Miltenyi Biotec, 130-096-495), CD8 + T cells isolation kit, mouse (Miltenyl Biotec, 130-104-075), RNA-quick purification kit (Shang Hai Yishan Biotechnology Co., LTD, ES-RN001), Anti-PE microbeads (Miltenyi Biotec, 130-048-801), CD326 (EpCAM) microbeads, mouse (Miltenyi Biotec, 130-105-958), Cytofix/CytopermTM fixation/permeabilization solution kit (BD Biosciences, 554714), TransScrip@ One-Step RT-PCR superMix (Transgene, AT411-02), Secrete-pair gaussia luciferase assay kit (GeneCopoeia, LF062), Duolink® in situ detection reagents red (Merck, DUO92008), Mouse C–C motif chemokine 4 (Ccl4) ELISA kit (4A Biotech, MOEB0088), Human C–C motif chemokine 4 (CCL4) ELISA kit (4A Biotech, HUEB0132), Mouse plasminogen activator inhibitor 2, macrophage (Serpinb2) ELISA kit (4A Biotech, MOEB1945), Human plasminogen activator inhibitor 2 (SerpinB2) ELISA Kit (4A Biotech, HUEB2195), MycoBlue Mycoplasma Detector (Vazyme, D101-02), Dual luciferase reporter assay system (Promega, E1910), ALDEFLUORTM Kit (STEMCELL Technologies, 01700). .. The following commercial kits were used: CFSE cell division tracker kit (Biolegend, 423801), Precision count beadsTM (Biolegend, 424902), SimpleChIP® plus Article Title: ID1 expressing macrophages support cancer cell stemness and limit CD8 + T cell infiltration in colorectal cancer. Article Snippet: The following chemicals and recombinant proteins were used: Y15 (Selleck, S5321), 5-Fluorouracil (5-FU) (Selleck, S1209), ML323 (Selleck, S7529), Propidium iodide (Solarbio, P8080), Matrigel (Corning, 354230), Fibronectin (Corning, 354008), StemXVivo serum-free tumorsphere media (R&D, CCM012), Ionomycin (Merck, IO634), GolgiStop (BD Biosciences, 554724), Brefeldin A solution (1,000X) Nature Communications | (2023) 14:7661 14 (Biolegend, 4200601), β-Mercaptoethanol (Solarbio, M8210), Human truStain FcXTM (Fc receptor blocking solution) (Biolegend, 422302), Recombinantmurine IL-4 (Peprotech, 214-14), Recombinantmurine IL2 (Peprotech, 212-12), Recombinant human IFN-γ (Peprotech, 300-02), Recombinant murine IFN-γ (Peprotech, 315-05), Recombinant murine m-CSF (Peprotech, 315-02), Leptomycin B (Selleck, S7580), Phorbol 12- myristate 13-acetate (PMA) (Merck, P8139), Collagenase IV (Merck, C4BIOC), Dnase I (Merck, 10104159001), Lithium phosphorus sulfide (LPS) powder (Merck, 916374), Puromycin (Invitrogen, A1113803), Fluorescent mounting medium with DAPI (ZSGB-BIO, ZLI-9557), Lymphocyte separation medium (Solarbio, P8900), 2,2,2-tribromoethanol (Merck, T48402), Protein A/G Plus-agarose (Santa Cruz, sc-2003), Ovalbumin (257-264) chicken (Merck, S7951), Verteporfin (Selleck, S1786). .. The following commercial kits were used: CFSE cell division tracker kit (Biolegend, 423801), Precision count beadsTM (Biolegend, 424902), SimpleChIP® plus Reporter Assay:Article Title: ID1 expressing macrophages support cancer cell stemness and limit CD8 + T cell infiltration in colorectal cancer Article Snippet: The following commercial kits were used: CFSE cell division tracker kit (Biolegend, 423801), Precision count beadsTM (Biolegend, 424902), SimpleChIP® plus sonication chromatin IP kit (Cell Signaling Technology, 56383), Four color labeled kit (TSA-RM) (PANOVUE Biotechnology Co.,LTD, 1100020), CD8 + T cell isolation kit, human (Miltenyi Biotec, 130-096-495), CD8 + T cells isolation kit, mouse (Miltenyl Biotec, 130-104-075), RNA-quick purification kit (Shang Hai Yishan Biotechnology Co., LTD, ES-RN001), Anti-PE microbeads (Miltenyi Biotec, 130-048-801), CD326 (EpCAM) microbeads, mouse (Miltenyi Biotec, 130-105-958), Cytofix/CytopermTM fixation/permeabilization solution kit (BD Biosciences, 554714), TransScrip@ One-Step RT-PCR superMix (Transgene, AT411-02), Secrete-pair gaussia luciferase assay kit (GeneCopoeia, LF062), Duolink® in situ detection reagents red (Merck, DUO92008), Mouse C–C motif chemokine 4 (Ccl4) ELISA kit (4A Biotech, MOEB0088), Human C–C motif chemokine 4 (CCL4) ELISA kit (4A Biotech, HUEB0132), Mouse plasminogen activator inhibitor 2, macrophage (Serpinb2) ELISA kit (4A Biotech, MOEB1945), Human plasminogen activator inhibitor 2 (SerpinB2) ELISA Kit (4A Biotech, HUEB2195), MycoBlue Mycoplasma Detector (Vazyme, D101-02), Dual luciferase reporter assay system (Promega, E1910), ALDEFLUORTM Kit (STEMCELL Technologies, 01700). .. The following commercial kits were used: CFSE cell division tracker kit (Biolegend, 423801), Precision count beadsTM (Biolegend, 424902), SimpleChIP® plus Article Title: ID1 expressing macrophages support cancer cell stemness and limit CD8 + T cell infiltration in colorectal cancer. Article Snippet: The following chemicals and recombinant proteins were used: Y15 (Selleck, S5321), 5-Fluorouracil (5-FU) (Selleck, S1209), ML323 (Selleck, S7529), Propidium iodide (Solarbio, P8080), Matrigel (Corning, 354230), Fibronectin (Corning, 354008), StemXVivo serum-free tumorsphere media (R&D, CCM012), Ionomycin (Merck, IO634), GolgiStop (BD Biosciences, 554724), Brefeldin A solution (1,000X) Nature Communications | (2023) 14:7661 14 (Biolegend, 4200601), β-Mercaptoethanol (Solarbio, M8210), Human truStain FcXTM (Fc receptor blocking solution) (Biolegend, 422302), Recombinantmurine IL-4 (Peprotech, 214-14), Recombinantmurine IL2 (Peprotech, 212-12), Recombinant human IFN-γ (Peprotech, 300-02), Recombinant murine IFN-γ (Peprotech, 315-05), Recombinant murine m-CSF (Peprotech, 315-02), Leptomycin B (Selleck, S7580), Phorbol 12- myristate 13-acetate (PMA) (Merck, P8139), Collagenase IV (Merck, C4BIOC), Dnase I (Merck, 10104159001), Lithium phosphorus sulfide (LPS) powder (Merck, 916374), Puromycin (Invitrogen, A1113803), Fluorescent mounting medium with DAPI (ZSGB-BIO, ZLI-9557), Lymphocyte separation medium (Solarbio, P8900), 2,2,2-tribromoethanol (Merck, T48402), Protein A/G Plus-agarose (Santa Cruz, sc-2003), Ovalbumin (257-264) chicken (Merck, S7951), Verteporfin (Selleck, S1786). .. The following commercial kits were used: CFSE cell division tracker kit (Biolegend, 423801), Precision count beadsTM (Biolegend, 424902), SimpleChIP® plus Immunoprecipitation:Article Title: Helicobacter pylori Exploit Short-Chain Fatty Acids-Induced CAPZA1 Overexpression to Emerge CD44v9-Positive Stemness Article Snippet: HDAC activity was measured using an HDAC Activity Assay kit (BioVision, Milpitas, CA, cat# K331). .. Immunoprecipitation was performed using a Simple Chromatin Immunoprecipitation (ChIP) Plus |